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Comparative gene expression of PSP-toxin producing and non-toxic [An article from: Environment International] | ![Comparative gene expression of PSP-toxin producing and non-toxic [An article from: Environment International]](http://ecx.images-amazon.com/images/I/51QY2BJNG1L._SL160_.jpg)
 enlarge | Authors: F. Pomati, R. Kellmann, R. Cavalieri, B.p. Burns
Publisher: Elsevier
Category: Book
Buy New: $10.95
Format: Html
Media: Digital
Pages: 5
ASIN: B000P6O3VG
Publication Date: August 1, 2006
Availability: Available for download now
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Product Description
This digital document is a journal article from Environment International, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Blooms of the freshwater cyanobacterium Anabaena circinalis are recognised as an important health risk worldwide due to the production of a range of toxins such as saxitoxin (STX) and its derivatives, also known as paralytic shellfish poisoning (PSP) toxins. In this study the transcriptional profile of PSP toxin-producing and non-toxic strains of A. circinalis was investigated by means of a DNA microarray approach. Additionally, gene expression was studied after exposure of toxic A. circinalis cultures to lidocaine hydrochloride at 1 @mM for 2 h. Under standard growth conditions, a limited number of putative toxic-strain distinctive DNA fragments, identified in previous studies, were preferentially expressed in toxic versus non-toxic strains. The same genes did not significantly change their expression after exposure to 1 @mM lidocaine, conditions previously shown to induce STX production in the cyanobacterium Cylindrospermopsis raciborskii T3. Lidocaine supplementation, however, enhanced the transcription of genes involved in physiological adaptive responses and bloom formation in cyanobacteria, such as the gas vesicle structural protein A and phycocyanin. The heat shock protein HSP-70 and the chlorophyll-a binding protein isiA were significantly repressed by lidocaine exposure. Stress response proteins and genes implicated in secondary metabolism were repressed, including phosphopantetheinyl transferases. The BGGM^1 DNA microarray, used in this study, was shown to be suitable for gene expression studies in cultured toxic cyanobacteria and allowed the analysis of gene transcripts associated with surface scum formation by toxic A. circinalis.
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